SKU: 48949952341

Human SPP24 (Secreted phosphoprotein 24) ELISA Kit

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Description

Human SPP24 (Secreted phosphoprotein 24) ELISA KitThe Human SPP24 (Secreted phosphoprotein 24) ELISA Kit measures SPP24 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SPP24 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated

The Human SPP24 (Secreted phosphoprotein 24) ELISA Kit measures SPP24 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SPP24 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with SPP24 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of SPP24 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 48949952341

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Serina L Crum
Lexington, US
★★★★★ 5
My dogs favorite toy
Size: Small
My dog loves these! She has a small mouth and not many balls fit in her mouth when we play fetch. The only thing is that she can't make the balls squeak on her own, it does take pressure. However, maybe that's a plus if you don't wanna listen to squeezing all day haha. They are durable, very bouncy even in the dirt. Great price fun toy!
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Reviewed in the United States on May 16, 2026
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Deborah B
Birmingham, US
★★★★★ 4
My dog loves them...but
Size: Small
The ball is firm, durable and bounces well, but the squeaker is a disappointment. My 16 lb chihuahua doxie mix is obsessed with squeaky toys. I bought the small size which is a good fit. These balls are a bit firm and hard to squeak but she is still able to squeak them endlessly. The squeaker only lasts a couple of weeks then it's just a ball that she ignores.
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Reviewed in the United States on May 11, 2026
M
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marge connolly
Birmingham, US
★★★★★ 5
Quality choice for my dog
Size: Small
These are great, great quality and a super great price
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Reviewed in the United States on May 26, 2026
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Verified Purchase
L. Holmquist
Grantham, US
★★★★★ 5
Small squeaky dog toy
Size: Small
My mini dachshunds love them, they fit in their mouth and they squeak🙂
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Reviewed in the United States on April 5, 2026
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Charndra
West Palm Beach, US
★★★★★ 5
Great toy!
Size: Small Size
My Frenchie absolutely loves this toy!!! It keeps her occupied for a long time. Has held up well to her chewing, and she's able to pick it up and carry it.
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Reviewed in the United States on March 24, 2026

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