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Description
Rat CYP17A1 (Steroid 17-alpha-hydroxylase) ELISA KitThe Rat (CYP17A1) Steroid 17 alpha hydroxylase ELISA Kit measures Steroid 17 alpha hydroxylase 17,20 lyase in Rat samples. The plate has been pre coated with Rat CYP17A1 antibody. CYP17A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CYP17A1 Antibody is added and binds to CYP17A1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CYP17A1 antibody. After incubation unbound
The Rat (CYP17A1) Steroid 17-alpha-hydroxylase ELISA Kit measures Steroid 17-alpha-hydroxylase/17,20 lyase in Rat samples. The plate has been pre-coated with Rat CYP17A1 antibody. CYP17A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CYP17A1 Antibody is added and binds to CYP17A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYP17A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CYP17A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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