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Description
Human GAPDH (Glyceraldehyde-3-phosphate Dehydrogenase) ELISA KitThe Human (GAPDH) Glyceraldehyde 3 phosphate Dehydrogenase ELISA Kit measures glyceraldehyde 3 phosphate dehydrogenase in samples. The plate has been pre coated with Human GAPDH antibody. GAPDH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GAPDH Antibody is added and binds to GAPDH in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GAPDH antibody. After incubation
The Human (GAPDH) Glyceraldehyde-3-phosphate Dehydrogenase ELISA Kit measures glyceraldehyde-3-phosphate dehydrogenase in samples. The plate has been pre-coated with Human GAPDH antibody. GAPDH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GAPDH Antibody is added and binds to GAPDH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GAPDH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GAPDH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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