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Description
Human IDH1 (Isocitrate Dehydrogenase, Nadp Cytoplasmic) ELISA KitThe Human (IDH1) Isocitrate Dehydrogenase, Nadp Cytoplasmic ELISA Kit measures Isocitrate dehydrogenase [NADP] cytoplasmic in samples. The plate has been pre coated with Human IDH1 antibody. IDH1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IDH1 Antibody is added and binds to IDH1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IDH1 antibody. After incubation
The Human (IDH1) Isocitrate Dehydrogenase, Nadp Cytoplasmic ELISA Kit measures Isocitrate dehydrogenase [NADP] cytoplasmic in samples. The plate has been pre-coated with Human IDH1 antibody. IDH1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IDH1 Antibody is added and binds to IDH1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IDH1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IDH1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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